Recombinant human SIRT2 protein (ab79948)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human SIRT2 protein
SIRT2 タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: 1.4 U/µg. One U =1 pmol/min.
Activity: =1.0 pmol/min/µg. Assay conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate, 500 µM NAD+ and Sirtuin 2 (125 - 8000 ng). Incubation condition: 30 min at 37°C followed by treatment with 50 µl of SIRT developer for 15 min at RT. Fluorescence intensity is measured at ex360/em460.
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精製度
> 90 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MASSLGSQKERLLDELTLEGVARYMQSERCRRVICLVGAGISTSAGIPDF RSPSTGLYDNLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYPGQFKPT ICHYFMRLLKDKGLLLRCYTQNIDTLERIAGLEQEDLVEAHGTFYTSHCV SASCRHEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSC MQSDFLKVDLLLVMGTSLQVQPFASLISKAPLSTPRLLINKEKAGQSDPF LGMIMGLGGGMDFDSKKAYRDVAWLGECDQGCLALAELLGWKKELEDLVR REHASIDAQSLEHHHHHH -
予測される分子量
36 kDa including tags -
領域
50 to 356 -
タグ
His tag C-Terminus -
配列の追加情報
NP_036369 36 kDa including tag
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab79948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- FLJ35621
- FLJ37491
- NAD dependent deacetylase sirtuin 2
see all -
機能
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
組織特異性
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
配列類似性
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
発生段階
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
翻訳後修飾
Phosphorylated at the G2/M transition of the cell cycle. -
細胞内局在
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab79948 は論文での使用が確認できていません。