製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human

関連製品

特性

Our Abpromise guarantee covers the use of ab82026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性1 unit equals 1 nanogram of purified protein.
  • アプリケーション

    SDS-PAGE

    EMSA

  • 精製度> 95 % SDS-PAGE.
    purified by affinity column
  • 製品の状態Liquid
  • 備考1 unit equals 1 nanogram of purified protein.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.90
    Constituents: 0.00584% EDTA, 0.476% HEPES, 0.0077% DTT, 0.55% Ammonium sulphate, 20% Glycerol

関連情報

  • 別名
    • Alternative splicing factor 1
    • Alternative-splicing factor 1
    • arginine/serine-rich 1
    • ASF
    • ASF 1
    • ASF-1
    • ASF1
    • FLJ53078
    • MGC5228
    • P33 subunit
    • Pre mRNA splicing factor SF2 P33 subunit
    • pre-mRNA-splicing factor SF2
    • Serine/arginine-rich splicing factor 1
    • SF2
    • SF2P33
    • SFRS1
    • Splicing factor
    • Splicing factor 2
    • Splicing factor 2 alternate splicing factor
    • Splicing factor arginine/serine rich 1
    • SR Splicing factor 1
    • SRp30a
    • srsf1
    • SRSF1_HUMAN
    see all
  • 機能Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors.
  • 配列類似性Belongs to the splicing factor SR family.
    Contains 2 RRM (RNA recognition motif) domains.
  • ドメインThe RRM 2 domain plays an important role in governing both the binding mode and the phosphorylation mechanism of the RS domain by SRPK1. RS domain and RRM 2 are uniquely positioned to initiate a highly directional (C-terminus to N-terminus) phosphorylation reaction in which the RS domain slides through an extended electronegative channel separating the docking groove of SRPK1 and the active site. RRM 2 binds toward the periphery of the active site and guides the directional phosphorylation mechanism. Both the RS domain and an RRM domain are required for nucleocytoplasmic shuttling.
  • 翻訳後修飾Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Phosphorylated by SRPK1 at multiple serines in its RS domain via a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds to a docking groove in the large lobe of the kinase domain of SRPK1 and this induces certain structural changes in SRPK1 and/or RRM 2 domain of SRSF1, allowing RRM 2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM 2, which then docks at the docking groove of SRPK1. This also signals RRM 2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed.
    Arg-97 is dimethylated, probably to asymmetric dimethylarginine.
  • 細胞内局在Cytoplasm. Nucleus speckle. In nuclear speckles. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt

Recombinant Human SF2 protein (ab82026) 使用論文

ab82026 has not yet been referenced specifically in any publications.

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