製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Wheat germ
  • アミノ酸配列
    • アクセッション番号Q01105
    • 生物種Human
    • 配列MSAPAAKVSKKELNSNHDGADETSEKEQQEAIEHIDEVQNEIDRLNEQAS EEILKVEQKYNKLRQPFFQKRSELIAKIPNFWVTTFVNHPQVSALLGEED EEALHYLTRVEVTEFEDIKSGYRIDFYFDENPYFENKVLSKEFHLNESGD PSSKSTEIKWKSGKDLTKRSSQTQNKASRKRQHEEPESFFTWFTDHSDAG ADELGEVIKDDIWPNPLQYYLVPDMDDEEGEGEEDDDDDEEEEGLEDIDE EGDEDEGEEDEDDDEGEEGEEDEGEDD
    • 分子量56 kDa including tags
    • 領域1 to 277

特性

Our Abpromise guarantee covers the use of ab114479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    ELISA

    SDS-PAGE

    Western blot

  • 製品の状態Liquid
  • 備考Protein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

関連情報

  • 別名
    • 2PP2A
    • HLA DR associated protein II
    • HLA-DR-associated protein II
    • I 2PP2A
    • I-2PP2A
    • I2PP2A
    • IGAAD
    • Inhibitor of granzyme A activated DNase
    • Inhibitor of granzyme A-activated DNase
    • Inhibitor of GzmA-activated DNase
    • inhibitor-2 of protein phosphatase-2A
    • IPP2A2
    • PHAPII
    • Phosphatase 2A inhibitor I2PP2A
    • protein phosphatase type 2A inhibitor
    • Protein SET
    • Set
    • SET nuclear oncogene
    • SET translocation
    • SET translocation (myeloid leukemia-associated)
    • SET_HUMAN
    • TAF I
    • TAF IBETA
    • TAF-I
    • TAFI
    • Template activating factor I
    • Template-activating factor I
    • Template-Activating Factor-I, chromatin remodelling factor
    see all
  • 機能Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
  • 組織特異性Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
  • 関連疾患Note=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.
  • 配列類似性Belongs to the nucleosome assembly protein (NAP) family.
  • ドメインThe C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity.
  • 翻訳後修飾Isoform 2 is phosphorylated on Ser-15 and Thr-23.
    Isoform 2 is acetylated on Lys-11.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
    N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated.
  • 細胞内局在Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum.
  • Information by UniProt

Recombinant Human SET protein 画像

  • 12.5% SDS-PAGE showing ab114479 at approximately 55.88kDa stained with Coomassie Blue.

Recombinant Human SET protein (ab114479) 使用論文

ab114479 has not yet been referenced specifically in any publications.

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