製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列Recombinant Human SCF is an 18.4 kDa protein containing 165 amino acid residues: MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYV PGMDVL PSHCWISEMV VQLSDSLTDLLDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIFNRSIDAF KDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA

関連製品

特性

Our Abpromise guarantee covers the use of ab9717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

    Western blot

  • エンドトキシン・レベル< 0.100 Eu/µg
  • 製品の状態Lyophilised
  • 備考The ED50, determined by the dose-dependent stimulation of the proliferation of the human TF-1 cells, is < 2.0 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • C kit ligand
    • C-kit ligand
    • Ckit ligand
    • DCUA
    • DFNA69
    • DKFZp686F2250
    • familial progressive hyperpigmentation 2
    • FPH2
    • FPHH
    • KIT ligand
    • Kitl
    • KITLG
    • KL 1
    • KL1
    • Mast cell growth factor
    • MGF
    • MGF stem cell factor
    • SCF
    • SCF_HUMAN
    • SF
    • SHEP7
    • sKITLG
    • Soluble KIT ligand
    • Steel factor
    • steel, mouse, homolog of
    • Stem cell factor
    • Stem cell factor precursor
    see all
  • 機能Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • 関連疾患Hyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric
  • 配列類似性Belongs to the SCF family.
  • 発生段階Acts in the early stages of hematopoiesis.
  • 翻訳後修飾A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.
  • 細胞内局在Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt

Recombinant human SCF protein 画像

  • Anti-SCF antibody (ab9716) at 0.1 µg/ml + Recombinant human SCF protein (ab9717) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Exposure time : 30 seconds

Recombinant human SCF protein (ab9717) 使用論文

ab9717 has not yet been referenced specifically in any publications.

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