Recombinant human p38 gamma/MAPK12 protein (ab125651)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human p38 gamma/MAPK12 protein
p38 gamma/MAPK12 タンパク質・ペプチド 製品一覧 -
生理活性
The specific activity of ab125651 was determined to be 220 nmol/min/mg.
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精製度
> 90 % Densitometry.
Purity was determined to be >90% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
71 kDa including tags -
領域
1 to 367
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特性
Our Abpromise guarantee covers the use of ab125651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- ERK 6
- ERK-6
- ERK6
see all -
機能
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. -
組織特異性
Highly expressed in skeletal muscle and heart. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
ドメイン
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻訳後修飾
Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. -
細胞内局在
Cytoplasm. Mitochondrion. Mitochondrial when associated with SH3BP5. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab125651 は 1 報の論文で使用されています。
- Koh A et al. Microbial Imidazole Propionate Affects Responses to Metformin through p38?-Dependent Inhibitory AMPK Phosphorylation. Cell Metab 32:643-653.e4 (2020). PubMed: 32783890