Recombinant human Rsk 2 / MAPKAP Kinase 1b protein (ab60881)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human Rsk 2 / MAPKAP Kinase 1b protein
Rsk 2 / MAPKAP Kinase 1b タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab60881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- 90 kDa ribosomal protein S6 kinase 3
- CLS
- HU 3
see all -
機能
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
組織特異性
Expressed in many tissues, highest levels in skeletal muscle. -
関連疾患
Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻訳後修飾
Autophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369. - Information by UniProt
画像
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The specific activity of Rsk 2 / MAPKAP Kinase 1b (ab60881) was determined to be 110 nmol/min/mg as per activity assay protocol and was equivalent to 157 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab60881
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SDS PAGE analysis of ab60881
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Sample Kinase Activity Plot.
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ab60881 on SDS-PAGE, MW ~112 kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab60881 は 1 報の論文で使用されています。
- Yang S & Counter CM Cell cycle regulated phosphorylation of the telomere-associated protein TIN2. PLoS One 8:e71697 (2013). PubMed: 23977114