製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 配列
      MGSSHHHHHHSSGLVPRGSHMAAIRKKLVVVGDGACGKTCLLIVFSKDEF PEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVI LMCFSVDSPDSLENIPEKWVPEVKHFCPNVPIILVANKKDLRSDEHVRTE LARMKQEPVRTDDGRAMAVRIQAYDYLECSAKTKEGVREVFETATRAALQ KRYGSQNGCINCC
    • 分子量
      24 kDa including tags
    • 領域
      1 to 193
    • タグ
      His tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab107139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Mass Spectrometry

  • 質量分析
    MALDI-TOF
  • 精製度
    > 90 % SDS-PAGE.
    ab107139 is purified using conventional chromatography techniques.
  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.2M Sodium chloride, 20mM Tris HCl, 2mM DTT, pH 8.0

関連情報

  • 別名
    • Aplysia RAS-related homolog 6
    • ARH6
    • ARHB
    • H6
    • MST081
    • MSTP081
    • oncogene RHO H6
    • ras homolog family member B
    • ras homolog gene family, member B
    • Rho cDNA clone 6
    • Rho related GTP binding protein RhoB Precursor
    • Rho-related GTP-binding protein RhoB
    • Rhob
    • RHOB_HUMAN
    • RHOH6
    see all
  • 機能
    Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.
  • 配列類似性
    Belongs to the small GTPase superfamily. Rho family.
  • 翻訳後修飾
    Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.
  • 細胞内局在
    Late endosome membrane. Cell membrane. Nucleus. Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.
  • Information by UniProt

画像

  • 15% SDS-PAGE showing ab107139 (3µg).
    Note: Molecular weight on SDS-PAGE will appear higher than predicted.

参考文献

This product has been referenced in:
  • Reichman M  et al. A High-Throughput Assay for Rho Guanine Nucleotide Exchange Factors Based on the Transcreener GDP Assay. J Biomol Screen 20:1294-9 (2015). Read more (PubMed: 26195453) »

See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab107139.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録