The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The deletion of 17 C terminal amino acids in RAP1B delta C leads to the loss of the phosphorylation site Ser179 and the geranyl geranylation site. Protein preparation is 53% GDP and 47% GTP loaded, measured by HPLC.
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Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.