製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P11233
    • 生物種Human
    • 配列MGSSHHHHHHSSGLVPRGSHMGSHMAANKPKGQNSLALHKVIMVGSGGVG KSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYA AIRDNYFRSGEGFLCVFSITEMESFAATADFREQILRVKEDENVPFLLVG NKSDLEDKRQVSVEEAKNRAEQWNVNYVETSAKTRANVDKVFFDLMREIR ARKMEDSKEKNGKKKRKSLAKRIRERC
    • 分子量26 kDa including tags
    • 領域1 to 203
    • タグHis tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab102555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    Mass Spectrometry

    SDS-PAGE

  • 質量分析
    MALDI-TOF
  • 精製度> 90 % SDS-PAGE.
    ab102555 was purified using conventional chromatography techniques.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 2mM DTT, 100mM Sodium chloride, 0.1mM PMSF, pH 8.0

関連情報

  • 別名
    • MGC48949
    • RAL
    • Ral a
    • Ral A protein
    • RALA
    • RALA_HUMAN
    • Ras family small GTP binding protein RALA
    • RAS like protein A
    • Ras related protein RalA
    • Ras-related protein Ral-A
    • v ral simian leukemia viral oncogene homolog A
    • v ral simian leukemia viral oncogene homolog A (ras related)
    see all
  • 機能Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.
  • 配列類似性Belongs to the small GTPase superfamily. Ras family.
  • 翻訳後修飾Prenylation is essential for membrane localization. The geranylgeranylated form and the farnesylated mutant does not undergo alternative prenylation in response to geranylgeranyltransferase I inhibitors (GGTIs) and farnesyltransferase I inhibitors (FTIs).
  • 細胞内局在Cell surface. Cell membrane. Cleavage furrow. Midbody. Prior to LPA treatment found predominantly at the cell surface and in the presence of LPA co-localizes with LPAR1 and LPAR2 in the endocytic vesicles. During early cytokinesis localizes at the cleavage furrow membrane. Colocalizes with EXOC2 at the early midbody ring and persists there till maturation of the midbody.
  • Information by UniProt

Recombinant Human RALA protein 画像

  • 15% SDS-PAGE analysis of 3µg of ab102555.
  • Anti-RALA antibody (ab96759) at 1/3000 dilution + Recombinant Human RALA protein (ab102555) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

Recombinant Human RALA protein (ab102555) 使用論文

ab102555 has not yet been referenced specifically in any publications.

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