Recombinant human Raf1 (mutated Y341 E + Y341 E) protein (ab55721)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human
    • 分子量63 kDa
    • 修飾mutated Y340 E + Y341 E
    • タグGST tag N-Terminus
    • 配列の追加情報Recombinant fragment, corresponding to amino acids 306-end of Human Raf 1, and containing the mutations Y340E Y341E.

特性

Our Abpromise guarantee covers the use of ab55721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific Activity: approximately 6000 nmol/min/mg.
  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 製品の状態Liquid
  • 備考

    It has been confirmed that ab55721 contains the mutations Y340E and Y341E by DNA sequencing.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • c Raf
    • C-raf
    • C-Raf proto-oncogene, serine/threonine kinase
    • CMD1NN
    • cRaf
    • Craf 1 transforming gene
    • Craf1 transforming gene
    • EC 2.7.11.1
    • kinase Raf1
    • Murine sarcoma 3611 oncogene 1
    • NS5
    • Oncogene MIL
    • Oncogene RAF1
    • OTTHUMP00000160218
    • OTTHUMP00000207813
    • OTTHUMP00000209389
    • Protein kinase raf 1
    • Proto-oncogene c-RAF
    • RAF
    • Raf 1
    • Raf 1 proto oncogene serine/threonine kinase
    • Raf proto oncogene serine/threonine protein kinase
    • RAF proto-oncogene serine/threonine-protein kinase
    • RAF-1
    • RAF1
    • RAF1_HUMAN
    • Similar to murine leukemia viral (V-raf-1) oncogene homolog 1
    • TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV
    • v raf 1 murine leukemia viral oncogene homolog 1
    • v-raf murine sarcoma viral oncogene homolog 1
    • vraf1 murine leukemia viral oncogene homolog 1
    see all
  • 機能Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
  • 組織特異性In skeletal muscle, isoform 1 is more abundant than isoform 2.
  • 関連疾患Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
    Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
  • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 RBD (Ras-binding) domain.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.
  • 細胞内局在Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
  • Information by UniProt

Recombinant human Raf1 (mutated Y341 E + Y341 E) protein 画像

  • Kinase activity assay using ab55721. Specific activity: approximately 6000 nmol/min/mg.
  • SDS-PAGE analysis of ab55721 with molecular weight markers. Approximate molecular weight: 63kDa

Recombinant human Raf1 (mutated Y341 E + Y341 E) protein (ab55721) 使用論文

ab55721 has not yet been referenced specifically in any publications.

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