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Our Abpromise guarantee covers the use of ab58738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This protein has been shown to stimulate protein synthesis in L6 myoblasts at an ED50 of ≤ 10 ng/ml.
R3 IGFI is a recombinant analog of insulin-like growth factor containing the complete human IGFI amino acid sequence with substitution of Arg for Glu 3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture. Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.
NOTE: This protein is full length, SwissProt ID P05019. The protein undergoes molecular processing and the IGF1 is full length at aa 49-118. The rest of the sequence is signal peptide and propeptide
Shipped at 4°C. Store at 4°C prior to reconstitution. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
The product is lyophilized from 0.1 M acetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab58738 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"