Recombinant human PYK2 protein (ab60878)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human
    • 領域360 to 690

関連製品

特性

Our Abpromise guarantee covers the use of ab60878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 製品の状態Liquid
  • 備考

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, 0.1mM PMSF, pH 7.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • CADTK
    • CAK-beta
    • CAKB
    • CAKbeta
    • Calcium regulated non receptor proline rich tyrosine kinase
    • Calcium-dependent tyrosine kinase
    • Cell adhesion kinase beta
    • E430023O05Rik
    • EC 2.7.10.2
    • FADK 2
    • FADK2
    • FAK2
    • FAK2_HUMAN
    • Focal adhesion kinase 2
    • MGC124628
    • PKB
    • Proline-rich tyrosine kinase 2
    • Protein kinase B
    • Protein Tyrosine Kinase 2 Beta
    • Protein-tyrosine kinase 2-beta
    • PTK
    • PTK2B
    • PTK2B protein tyrosine kinase 2 beta
    • PYK2
    • RAFTK
    • RAFTK2
    • Related adhesion focal tyrosine kinase
    see all
  • 機能Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
  • 組織特異性Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
  • 細胞内局在Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
  • Information by UniProt

Recombinant human PYK2 protein 画像

  • ab60878 on SDS-PAGE, MW ~39 kDa.
  • Kinase activity plot

Recombinant human PYK2 protein (ab60878) 使用論文

ab60878 has not yet been referenced specifically in any publications.

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Thank you for contacting us. I do want to apologize for the tardiness of this response.

I have been in contact with the originator labs for ab42622 and ab60878 PYK2 proteins, in regards to whether these products are phosphorylated at Y402. U...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"