The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
14% SDS-PAGE showing ab104361 at approximately 12.48kDa:
Lane 1: M.W. marker – 14, 21, 31, 45, 66, 97 kDa
Lane 2: reduced and boiled sample, 5µg/lane.
Lane 3: non-reduced and non-boiled sample, 5µg/lane.
has not yet been referenced specifically in any publications.