Recombinant human Prolyl Endopeptidase protein (ab80376)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列MHHHHHHLSLQYPDVYRDETAVQDYHGHKICDPYAWLEDPDSEQTKAFVE AQNKITVPFLEQCPIRGLYKERMTELYDYPKYSCHFKKGKRYFYFYNTGL QNQRVLYVQDSLEGEARVFLDPNILSDDGTVALRGYAFSEDGEYFAYGLS ASGSDWVTIKFMKVDGAKELPDVLERVKFSCMAWTHDGKGMFYNSYPQQD GKSDGTETSTNLHQKLYYHVLGTDQSEDILCAEFPDEPKWMGGAELSDDG RYVLLSIREGCDPVNRLWYCDLQQESSGIAGILKWVKLIDNFEGEYDYVT NEGTVFTFKTNRQSPNYRVINIDFRDPEESKWKVLVPEHEKDVLEWIACV RSNFLVLCYLHDVKNILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEI FYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPS KDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRH MGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTS PKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHA WTTDYGCSDSKQHFEWLVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRV VPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVS DMFAFIARCLNVDWIP
    • 分子量82 kDa
    • 領域2 to 710

特性

Our Abpromise guarantee covers the use of ab80376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    Specific Activity: ≥ 12 pmol/min/µg. Assay condition: 50 mM Tris (pH 7.5), 100 µg BSA, 20 µM substrate and 500 ng Prolyl Endopeptidase protein at room temperature for 30 min.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 精製度> 90 % SDS-PAGE.

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • dJ355L5.1 (prolyl endopeptidase)
    • HGNC:9358
    • MGC16060
    • OTTHUMP00000040498
    • PE
    • PEP
    • Post proline cleaving enzyme
    • Post-proline cleaving enzyme
    • PPCE_HUMAN
    • Prep
    • Prolyl endopeptidase
    • Prolyl oligopeptidase
    see all
  • 機能Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.
  • 配列類似性Belongs to the peptidase S9A family.
  • 翻訳後修飾The N-terminus is blocked.
  • 細胞内局在Cytoplasm.
  • Information by UniProt

Recombinant human Prolyl Endopeptidase protein 画像

  • Lane 1: ab80376 on 10% SDS-PAGE, Coomassie staining, 4µg.
    Lane 2: Protein marker.

Recombinant human Prolyl Endopeptidase protein (ab80376) 使用論文

ab80376 has not yet been referenced specifically in any publications.

Product Wall



Assay condition: 50 mM Tris (pH 7.5), 0.1% BSA, 20 uM substrate (A-P-AMC), and 500 ng ab80376 at room temperature for 30 min.

I hope this is helpful. Please feel free to contact me again with any further questions.


We don’t think POP can cleave BSA. See below for the answer. Some information taken from references.

In humans prolyl endopeptidase is encoded by the PREP gene.[5][6] The protein encoded by this gene is a cytosolic prolyl endopeptidase ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"