Recombinant human Procathepsin K protein (ab123765)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P43235
    • 生物種Human
    • 分子量35 kDa
    • 領域19 to 329

関連製品

特性

Our Abpromise guarantee covers the use of ab123765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性SPECFIC ACTIVITY: After auto-catalytical activation at pH 4.0 the specific activity is >1000 mU/mg (international unit 1µmole/min/mg). ACTIVATION: Procathepsin K can be activated by adjusting the pH 4.0 by adding an equal volume of 100mM NaAc pH 3.9, 10 mM DTT, 5 mM EDTA followed by incubation for 40 min at RT. Activated mature Cathepsin is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, we recommend to add methyl methanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) and to freeze the sample in liquid nitrogen or on dry ice. The hydrophobic thiol-reactive compound MMTS modifies cysteine’s by attaching its relatively small, uncharged thiomethyl-blocking group to reactive sulfhydryl groups. This reversible reaction arrests the autoproteolytic process. The activity of the enzyme can be restored to nearly unmodified levels by adding L-cysteine (3M excess over MMTS) to the enzyme solution.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Tris, 2.92% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Cathepsin K
    • Cathepsin O
    • Cathepsin O1
    • Cathepsin O2
    • Cathepsin X
    • CATK_HUMAN
    • Ctsk
    • CTSO
    • CTSO2
    • PKND
    • PYCD
    see all
  • 機能Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
  • 組織特異性Predominantly expressed in osteclasts (bones).
  • 関連疾患Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.
  • 配列類似性Belongs to the peptidase C1 family.
  • 細胞内局在Lysosome.
  • Information by UniProt

Recombinant human Procathepsin K protein 画像

  • SDS-PAGE showing ab123765.

Recombinant human Procathepsin K protein (ab123765) 使用論文

ab123765 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your call and email.

I am very sorry to confirm that it is not possible for us to custom make a Procathepsin K protein to your specifications.

When I google "custom made proteins with isotope labeled amino acids&q...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"