Recombinant human Procalcitonin protein (ab92843)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列APFRSALESS PADPATLSED EARLLLAALV QDYVQMKASE LEQEQEREGS SLDSPRSKRC GNLSTCMLGT YTQDFNKFHT FPQTAIGVGA PGKKRDMSSD LERDHRPHVS MPQNAN

特性

Our Abpromise guarantee covers the use of ab92843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Bioassay: Human Procalcitonin was tested using Biomerieux reagents on the Min-Vidas. The assay was run at 80 ng/ml with a recovery of 82 percent.
  • アプリケーション

    SDS-PAGE

    Western blot

  • 精製度> 95 % SDS-PAGE.
    Purity is approximately 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE.
  • 製品の状態Lyophilised
  • 備考Bioassay: Human Procalcitonin was tested using Biomerieux reagents on the Min-Vidas. The assay was run at 80 ng/ml with a recovery of 82 percent.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10mM Sodium phosphate, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Reconstitute at 0.1 mg/ml with sterile water. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

関連情報

  • 別名
    • CALC1
    • CALCA
    • calcitonin related polypeptide alpha
    • CGRP
    • CGRP1
    • CT
    • katacalcin
    • KC
    see all
  • 関連性Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis.
  • 細胞内局在Secreted

Recombinant human Procalcitonin protein 画像

  • Anti-Procalcitonin antibody [13F2] (ab90489) at 1 µg/ml + Recombinant human Procalcitonin protein (ab92843) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 3 minutes
  • ab92843 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human Procalcitonin has a predicted MW of 12.8 kDa.

Recombinant human Procalcitonin protein (ab92843) 使用論文

ab92843 has not yet been referenced specifically in any publications.

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Thank you for contacting us.
The shipment of the package should not have affected the activity of the antibody. Lyophilised protein is stable at room temperature for days. I am unsure as to why you have seen and increase in signal in this case, bu...

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Thank you for your enquiry. I appreciate your patience, it has regrettably taken some time to confirm the information for you.

I can let you know that these three proteins do not have a tag of any kind.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"