Recombinant Human Prealbumin protein (ab92931)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列MGPTGTGESK CPLMVKVLDA VRGSPAINVA VHVFRKAADD TWEPFASGKT SESGELHGLT TEEEFVEGIY KVEIDTKSYW KALGISPFHE HAEVVFTAND SGPRRYTIAA LLSPYSYSTT AVVTNPKE

特性

Our Abpromise guarantee covers the use of ab92931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.
    Purified using conventional chromatography.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, PBS, pH 7.4

関連情報

  • 別名
    • Amyloid polyneuropathy
    • Amyloidosis I
    • ATTR
    • Carpal tunnel syndrome 1
    • CTS
    • CTS1
    • Dysprealbuminemic euthyroidal hyperthyroxinemia
    • Dystransthyretinemic hyperthyroxinemia
    • Epididymis luminal protein 111
    • HEL111
    • HsT2651
    • PALB
    • Prealbumin
    • Prealbumin amyloidosis type I
    • Prealbumin Thyroxine-binding
    • Senile systemic amyloidosis
    • TBPA
    • Thyroxine binding prealbumin
    • Transthyretin
    • TTHY_HUMAN
    • TTR
    • TTR protein
    see all
  • 機能Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • 組織特異性Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
  • 関連疾患Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
    Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
    Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
  • 配列類似性Belongs to the transthyretin family.
  • ドメインEach monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
  • 細胞内局在Secreted. Cytoplasm.
  • Information by UniProt

Recombinant Human Prealbumin protein 画像

  • 15% SDS-PAGE analysis of 3µg ab92931

Recombinant Human Prealbumin protein (ab92931) 使用論文

ab92931 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
We have used this product as a standard for Western blotting experiments examining prealbumin levels in biological fluids. Using native electrophoresis, we find that the protein runs to the expected molecular weight of approximately 55 kDa. Comparison to plasma-purified prealbumin shows a slight difference in migration, which is likely due to post-translational modifications not found on the recombinant protein. The protein has shown good stability following storage at -80 and also runs to the expected molecular weight during SDS-PAGE separation.

Electrophoretic separation shown in the figure was performed on 4-16% native Tris-Glycine gels. Proteins were subsequently transferred to PVDF membrane and blotted with anti-Prealbumin antibody [EP2929Y] (ab75815) at a dilution of 1:5,000. Bands were visualized with a goat-anti-rabbit IR Dye 800 secondary antibody at a 1:10,000 dilution. The image was collected on a Licor Odyssey CLx instrument.
Username

Dr. Sam Nowitzki

Verified customer

投稿 Jul 09 2015

Thank you for contacting us. I contacted the laboratory regarding your size issue and got the following responseThe size of our Prealbumin, 21-147aa, Human, Recombinant, E.coli is 13.8kDa. The small difference could be attributed to standard deviation...

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