Recombinant human PRAK/MK5 protein (ab125743)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human PRAK/MK5 protein
PRAK/MK5 タンパク質・ペプチド 製品一覧 -
生理活性
The specific activity of ab125743 was determined to be 172 nmol/min/mg.
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精製度
> 80 % Densitometry.
Purity was determined to be >80% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
79 kDa including tags -
領域
1 to 471
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関連製品
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Related Products
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab125743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
ab204861 (Hsp27 peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as PRAK
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- MAP kinase-activated protein kinase 5
- MAPK-activated protein kinase 5
- MAPK5_HUMAN
see all -
機能
Mediates stress-induced small heat shock protein 27 phosphorylation. -
組織特異性
Expressed ubiquitously. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12. -
細胞内局在
Cytoplasm. Nucleus. Also observed in the nucleus. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab125743 は論文での使用が確認できていません。