Recombinant human PPP1A/PPP1CA protein (ab113150)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS, Functional Studies
製品の詳細
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製品名
Recombinant human PPP1A/PPP1CA protein
PPP1A/PPP1CA タンパク質・ペプチド 製品一覧 -
生理活性
>3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37 °C, pH 7.5, using 10 mM of substrate.
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精製度
> 80 % SDS-PAGE.
ab113150 was purified using conventional chromatography techniques. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
40 kDa including tags -
領域
1 to 330 -
タグ
His tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab113150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Mass Spectrometry
Functional Studies
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質量分析
MALDI-TOF -
製品の状態
Liquid -
備考
This product was previously labelled as PPP1A
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.5
Constituents: 0.002% PMSF, 0.02% DTT, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.01% Manganese chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit
- Catalytic subunit
- EC 3.1.3.16
see all -
機能
Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. -
配列類似性
Belongs to the PPP phosphatase family. PP-1 subfamily. -
細胞内局在
Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab113150 は論文での使用が確認できていません。