Recombinant human PPM1G protein (ab109936)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号O15355
    • 生物種Human
    • 配列MGSSHHHHHHSSGLVPRGSHMGAYLSQPNTVKCSGDGVGAPRLPLPYGFS AMQGWRVSMEDAHNCIPELDSETAMFSVYDGHGGEEVALYCAKYLPDIIK DQKAYKEGKLQKALEDAFLAIDAKLTTEEVIKELAQIAGRPTEDEDEKEK VADEDDVDNEEAALLHEEATMTIEELLTRYGQNCHKGPPHSKSGGGTGEE PGSQGLNGEAGPEDSTRETPSQENGPTAKAYTGFSSNSERGTEAGQVGEP GIPTGEAGPSCSSASDKLPRVAKSKFFEDSEDESDEAEEEEEDSEECSEE EDGYSSEEAENEEDEDDTEEAEEDDEEEEEEMMVPGMEGKEEPGSDSGTT AVVALIRGKQLIVANAGDSRCVVSEAGKALDMSYDHKPEDEVELARIKNA GGKVTMDGRVNGGLNLSRAIGDHFYKRNKNLPPEEQMISALPDIKVLTLT DDHEFMVIACDGIWNVMSSQEVVDFIQSKISQRDENGELRLLSSIVEELL DQCLAPDTSGDGTGCDNMTCIIICFKPRNTAELQPESGKRKLEEVLSTEG AEENGNSDKKKKAKRD
    • 分子量61 kDa including tags
    • 領域1 to 546
    • タグHis tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab109936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37°C, pH 7.5 using 10mM of substrate.
  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 精製度> 85 % SDS-PAGE.
    ab109936 is purified using conventional chromatography techniques.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride, 0.02% DTT, 0.002% PMSF

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • MGC1675
    • MGC2870
    • OTTHUMP00000123426
    • PP2C
    • PP2C gamma
    • PP2C-gamma
    • PP2CG
    • PP2Cgamma
    • PPM1G
    • PPM1G_HUMAN
    • PPP2CG
    • Protein phosphatase 1C
    • Protein phosphatase 1G
    • Protein phosphatase 2 catalytic subunit gamma isoform
    • Protein phosphatase 2C gamma isoform
    • Protein phosphatase 2C isoform gamma
    • Protein phosphatase magnesium dependent 1 gamma
    • Protein phosphatase magnesium-dependent 1 gamma
    • Protein phosphatase Mg2+/Mn2+ dependent 1G
    see all
  • 組織特異性Widely expressed. Most abundant in testis, skeletal muscle, and heart.
  • 配列類似性Belongs to the PP2C family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm.
  • Information by UniProt

Recombinant human PPM1G protein 画像

  • 15% SDS-PAGE showing ab109936 (3µg).

Recombinant human PPM1G protein (ab109936) 使用論文

ab109936 has not yet been referenced specifically in any publications.

Product Wall

Here is the assay protocolused to assess protein activityfor ab109936:

1. Prepare a 200ul reaction mix into a suitable container : The final concentrations are 10mM Tris(pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP.
2. Pre-warm react...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"