製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列MGSSHHHHHHSSGLVPRGSHMGKRQHQKDKMYITCAEYTHFYGGKKPDLP QTNFRRLPFDHCSLSLQPFVYPVCTPDGIVFDLLNIVPWLKKYGTNPSNG EKLDGRSLIKLNFSKNSEGKYHCPVLFTVFTNNTHIVAVRTTGNVYAYEA VEQLNIKAKNFRDLLTDEPFSRQDIITLQDPTNLDKFNVSNFYHVKNNMK IIDPDEEKAKQDPSYYLKNTNAETRETLQELYKEFKGDEILAATMKAPEK KKVDKLNAAHYSTGKVSASFTSTAMVPETTHEAAAIDEDVLRYQFVKKKG YVRLHTNKGDLNLELHCDLTPKTCENFIRLCKKHYYDGTIFHRSIRNFVI QGGDPTGTGTGGESYWGKPFKDEFRPNLSHTGRGILSMANSGPNSNRSQF FITFRSCAYLDKKHTIFGRVVGGFDVLTAMENVESDPKTDRPKEEIRIDA TTVFVDPYEEADAQIAQERKTQLKVAPETKVKSSQPQAGSQGPQTFRQGV GKYINPAATEQQRKSPQPVPLSPCPRRSPVGVLGTSAPGSSRLPDDH
    • 領域1 to 527

特性

Our Abpromise guarantee covers the use of ab93731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific activity is > 290 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant PPIL2 protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.
    Purified using conventional chromatography techniques.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • FLJ39930
    • CYC4
    • Cyclophilin 60
    • Cyclophilin 60kDa
    • Cyclophilin like protein Cyp 60
    • Cyclophilin-60
    • Cyclophilin-like protein Cyp-60
    • CYP60
    • hCyP 60
    • MGC33174
    • MGC787
    • Peptidyl prolyl cis trans isomerase like 2
    • Peptidyl-prolyl cis-trans isomerase-like 2
    • Peptidylprolyl cis trans isomerase
    • Peptidylprolyl isomerase cyclophilin like 2
    • PPIase
    • PPIL2
    • PPIL2_HUMAN
    • Rotamase
    • Rotamase PPIL2
    see all
  • 機能PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • 組織特異性Highest expression in thymus, pancreas and testis. Also detected in heart, placenta, lung, liver, skeletal muscle, kidney, spleen, prostate, ovary, small intestine and colon. Poorly detected in brain and leukocytes. Strong protein expression in lymph node (cortical, paracortical and medullar regions), thyroid (follicular epithelial cells), testis (developing spermatozoa), stomach (cells lining the gastric pit), pancreas, kidney (proximal and distal-tubule cells and collecting duct cells but not in glomeruli), endometrium and colon (goblet cells). Moderate protein expression in spleen, prostate (epithelium and squamous cell carcinomas), placenta and adrenal gland. Weak protein expression in liver, heart, breast, ovary, and lung. No protein expression in brain and bladder. High protein expression in most lymphomas and melanomas.
  • 配列類似性Belongs to the cyclophilin-type PPIase family. PPIL2 subfamily.
    Contains 1 PPIase cyclophilin-type domain.
    Contains 1 U-box domain.
  • 細胞内局在Nucleus.
  • Information by UniProt

Recombinant human PPIL2 protein 画像

  • 15% SDS Page of 3ug ab93731

Recombinant human PPIL2 protein (ab93731) 使用論文

ab93731 has not yet been referenced specifically in any publications.

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