製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号O00444
    • 生物種Human
    • 分子量140 kDa including tags
    • 領域1 to 836

特性

Our Abpromise guarantee covers the use of ab125558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The specific activity of ab125558 is 5.6 nmol/min/mg.
  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 精製度> 70 % Densitometry.
    ab125558 is >70% pure.
  • 製品の状態Liquid
  • 備考

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • PLK 4
    • PLK-4
    • PLK4
    • PLK4_HUMAN
    • Polo like Kinase 4
    • Polo-like kinase 4
    • Protein serine/threonine kinase
    • SAK
    • Serine/threonine kinase
    • Serine/Threonine Kinase 18
    • Serine/threonine protein kinase 18
    • Serine/threonine protein kinase PLK4
    • Serine/threonine protein kinase Sak
    • Serine/threonine-protein kinase 18
    • Serine/threonine-protein kinase PLK4
    • Serine/threonine-protein kinase SAK
    • Snk Akin Kinase
    • STK 18
    • STK18
    see all
  • 機能Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 1 POLO box domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Ubiquitinated; leading to its degradation by the proteasome.
    Tyrosine-phosphorylated by TEC.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome > centriole. Nucleus > nucleolus. Cleavage furrow. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.
  • Information by UniProt

Recombinant human PLK4 protein 画像

  • The specific activity of ab125558 was determined to be 5.6 nmol/min/mg.
  • SDS PAGE analysis of ab125558 (PLK4 protein).

Recombinant human PLK4 protein (ab125558) 使用論文

ab125558 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"