The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生理活性Activity: 500 U/mg. One unit is defined as the amount of enzyme that will
phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP.
備考Expressed in a Bacuovirus infected Sf9 expression system. Expected molecular weight of 36.7 kDa.
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Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
機能Serine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein.
組織特異性Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. Contains 2 POLO box domains. Contains 1 protein kinase domain.
翻訳後修飾Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.