Recombinant Human PLGF protein (ab84148)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Human PLGF protein
PLGF タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE. -
発現系
HEK 293 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
Theoretical Sequence: LPAVPPQQWALSAGNGSSEVEVVPFQEVWGRSYCRALER LVDVVSEYP SEVEHMFSPSCVSLLRCTGCCGDENLHCVPVETANVTMQ LLKIRSGDR PSYVELTFSQHVRCECRPLREKMKPERCGDAVPRR
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab84148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 1% Human serum albumin, 10% Trehalose
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再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
関連情報
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別名
- D12S1900
- Pgf
- PGFL
see all -
機能
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. -
組織特異性
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. -
配列類似性
Belongs to the PDGF/VEGF growth factor family. -
ドメイン
Isoform PlGF-2 contains a basic insert which acts as a cell retention signal. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. - Information by UniProt
画像
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Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein. -
1D SDS-PAGE of ab84148 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab84148
Lane 2: ab84148 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab84148 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
10μg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab84148 は論文での使用が確認できていません。