Recombinant Human PLA2G1B protein (ab63842)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P04054
    • 生物種Human
    • 配列MRGSHHHHHH GMASHMAVWQ FRKMIKCVIP GSDPFLEYNN YGCYCGLGGS GTPVDELDKC CQTHDNCYDQ AKKLDSCKFL LDNPYTHTYS YSCSGSAITC SSKNKECEAF ICNCDRNAAI CFSKAPYNKA HKNLDTKKYC QS
    • 領域23 to 148
    • タグHis tag N-Terminus
    • 配列の追加情報Total 142 AA. MW: 16 kDa (calculated). N-Terminal His-tag, 16 extra AA

特性

Our Abpromise guarantee covers the use of ab63842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Lyophilised
  • 備考Product is NOT sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    0.05M Acetate buffer pH 4.0

  • 再構成Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.

関連情報

  • 別名
    • Group IB phospholipase A2
    • Group IB secretory phospholipase A2
    • MGC119834
    • MGC119835
    • PA21B_HUMAN
    • Phosphatidylcholine 2 acylhydrolase
    • Phosphatidylcholine 2-acylhydrolase 1B
    • Phospholipase A2
    • Phospholipase A2 group IB
    • Phospholipase A2 group IB (pancreas)
    • PLA 2
    • PLA2
    • PLA2A
    • PLA2G1B
    • PPLA 2
    • PPLA2
    • sPLA2IB
    see all
  • 機能PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
  • 配列類似性Belongs to the phospholipase A2 family.
  • 細胞内局在Secreted.
  • Information by UniProt

Recombinant Human PLA2G1B protein 画像

  • 12% SDS-PAGE separation of ab63842. Molecular Weight: ~16 kDa.

    Lane 1. Molecular weight marker
    Lane 2. reduced and heated sample, 5µg/lane
    Lane 3. non-reduced and non-heated sample, 5µg/lane

Recombinant Human PLA2G1B protein (ab63842) 使用論文

ab63842 has not yet been referenced specifically in any publications.

Product Wall

Thank you for providing that information and sorry for the delay in getting back to you.
The information provided has been very useful and I will look into whether these antibodies would be likely to detect the protein and what may be contributing...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"