Recombinant human PKN3 protein (ab125615)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human PKN3 protein -
生理活性
The specific activity of ab125615 was determined to be 3 nmol/mg/min.
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精製度
> 75 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
130 kDa including tags -
領域
1 to 889 -
タグ
GST tag N-Terminus
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特性
Our Abpromise guarantee covers the use of ab125615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as PKN beta
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- pkn3
- PKN3_HUMAN
- PKNBETA
see all -
機能
Contributes to invasiveness in malignant prostate cancer. -
組織特異性
Expressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 3 REM (Hr1) repeats. -
ドメイン
The C1 domain does not bind the diacylglycerol (DAG). -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Nucleus. Cytoplasm > perinuclear region. Nuclear and perinuclear Golgi region. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab125615 は論文での使用が確認できていません。