Recombinant human PKC mu/PKD protein (ab60873)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, WB
製品の詳細
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製品名
Recombinant human PKC mu/PKD protein -
精製度
> 90 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human
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関連製品
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Related Products
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab60873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
Western blot
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製品の状態
Liquid -
備考
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as PKC mu
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- KPCD1_HUMAN
- nPKC D1
- nPKC mu
see all -
機能
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
細胞内局在
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation. - Information by UniProt
画像
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The specific activity of PKC mu/PKD (ab60873) was determined to be 650 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab60873
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SDS PAGE analysis of ab60873
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Ab5283 recognizes the full length tagged recombinant PKC mu protein (ab60873) which has an expected molecular weight of 131 kDa.
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Sample Kinase Activity Plot.
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ab60873 on SDS-PAGE, MW ~131 kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab60873 は論文での使用が確認できていません。