Recombinant human PKA beta (catalytic subunit) protein (ab60837)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human

特性

Our Abpromise guarantee covers the use of ab60837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

    Other

    Western blot

  • 精製度> 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • 製品の状態Liquid
  • 備考

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • cAMP-dependent protein kinase catalytic beta subunit isoform 4ab
    • cAMP-dependent protein kinase catalytic subunit beta
    • KAPCB_HUMAN
    • PKA C beta
    • PKA C-beta
    • PKACB
    • Prkacb
    • protein kinase A catalytic subunit beta
    • Protein kinase cAMP dependent catalytic beta
    see all
  • 機能Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.
  • 組織特異性Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain.
  • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.
  • 細胞内局在Cytoplasm. Nucleus. Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm.
  • Information by UniProt

Recombinant human PKA beta (catalytic subunit) protein 画像

  • Sample Kinase Activity Plot.
  • ab60837 on SDS-PAGE, MW ~65 kDa.
  • All lanes : Anti-PKA beta (catalytic subunit) antibody (ab94612) at 1 µg/ml

    Lane 1 : Recombinant human PKA beta (catalytic subunit) protein (ab60837) at 0.1 µg
    Lane 2 : Recombinant human PKA beta (catalytic subunit) protein (ab60837) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Recombinant human PKA beta (catalytic subunit) protein (ab60837) 使用論文

ab60837 has not yet been referenced specifically in any publications.

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Thank you for your inquiry.

I can confirm that ab60837 was used in a kinase assay with CREBtide synthetic peptide substrate (KRREILSRRPSYR) , stock 1mg/ml.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"