Recombinant human Pin1 protein (ab51230)

製品の概要

  • 製品名Recombinant human Pin1 protein
  • タンパク質長Full length protein

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQG EPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGE EDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPV FTDSGIHIILRTE

特性

Our Abpromise guarantee covers the use of ab51230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific activity is > 330 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity Assay

    1. 1. Prepare 180ul assay buffer into a suitable container: 133mM Tris-HCl, pH8.0, 5.5nM chymotrypsin, and 0.5ug of Pin1 recombinant protein.
    2. For blanks, load 133mM Tris-HCl, pH8.0 and 5.5nM chymotrypsin. (Enzyme dilution buffer: 133mM Tris-HCl, pH8.0)
    3. 2. Add 20ul of 5mM suc-AAFP-pNA to each well.
    4. (Substrate has to be dissolved in buffer of 460mM Lithium chloride in 3mM 2,2,2-Trifluoroethnol)
    5. 3. Incubated at room temperature for 10minutes.
    6. 4. Record the increase in A405 nm for 30 minutes at 37°C.
  • Note: Specific activity is lot specific and is always > 330 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. Please inquire about lot specific activity
  • アプリケーション

    Functional Studies

    SDS-PAGE

  • エンドトキシン・レベル< 1.000 Eu/µg
  • 質量分析
    MALDI-TOF
  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.1M Sodium chloride, 5mM DTT, 20mM Tris HCl, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • DOD
    • DODO, Drosophila, homolog of
    • FLJ40239
    • FLJ77628
    • MGC10717
    • NIMA interacting 1
    • Peptidyl prolyl cis trans isomerase NIMA interacting 1
    • Peptidyl prolyl cis/trans isomerase NIMA interacting
    • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
    • Peptidyl-prolyl cis-trans isomerase pin1
    • Peptidylprolyl cis/trans isomerase NIMA interacting 1
    • Pin 1
    • Pin1
    • PIN1_HUMAN
    • PPIase Pin1
    • Prolyl isomerase
    • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1
    • Protein NIMA interacting 1
    • Rotamase Pin1
    • UBL 5
    • UBL5
    see all
  • 機能Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
  • 配列類似性Contains 1 PpiC domain.
    Contains 1 WW domain.
  • ドメインThe WW domain is required for the interaction with STIL and KIF20B.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt

Recombinant human Pin1 protein 画像

  • ab51230 run on a 14% SDS-PAGE gel with molecular weight markers.

Recombinant human Pin1 protein (ab51230) 使用論文

ab51230 has not yet been referenced specifically in any publications.

Product Wall

You can dilute the protein in the same storage buffer in which the product is received. This buffer contains:
Preservative: None
Constituents: 20% Glycerol, 0.1M Sodium chloride, 5mM DTT, 20mM Tris HCl, pH 7.5
Please remember to keep freeze...

Read More

The product ab51230 does not come with an assay kit and we unfortunately do not have one in our catalogue. If you follow the instructions on the website though, you should be able to perform the assay.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"