The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity: 7.9 pmol/min/µg.
A 50 µl kinase reaction is conducted in a buffer containing 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 5 mM MgCl2, 5 µM ATP, 17 µg Phosphoinositol-4,5-Bisphosphate, and 150 ng enzyme for 90 minutes at room temperature.
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Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
PI3 kinase p110 subunit beta
PI3 kinase p85 subunit alpha
PtdIns 3 kinase p110
PtdIns 3 kinase p85 alpha
The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (p85).
SDS-PAGE - PIK3CB + PIK3R1 protein (Active) (ab80378)
Lane 1: ab80378 on 10% SDS-PAGE, Coomassie staining, 2µg. Lane 2: Protein marker.
Functional Studies - PIK3CB + PIK3R1 protein (Active) (ab80378)
has not yet been referenced specifically in any publications.