Recombinant human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein (ab91093)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human

特性

Our Abpromise guarantee covers the use of ab91093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The specific activity of PI 3 Kinase p110alpha + PI 3 kinase p85 alpha was determined to be 50 nm/min/mg as per activity assay protocol.
  • アプリケーション

    SDS-PAGE

    Western blot

    Functional Studies

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 2mM DTT, 0.1mM PMSF, pH 7.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • GRB1
    • MGC142161
    • MGC142163
    • p110 alpha
    • p85
    • p85 alpha
    • Phosphatidylinositol 3 kinase 85 kDa regulatory subunit alpha
    • Phosphatidylinositol 3 kinase associated p 85 alpha
    • Phosphatidylinositol 3 kinase catalytic 110 KD alpha
    • Phosphatidylinositol 3 kinase catalytic alpha polypeptide
    • Phosphatidylinositol 3 kinase regulatory 1
    • Phosphatidylinositol 3 kinase regulatory subunit alpha
    • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform
    • Phosphoinositide 3 kinase catalytic alpha polypeptide
    • Phosphoinositide 3 kinase regulatory subunit 1
    • Phosphoinositide 3 kinase regulatory subunit 1 (alpha)
    • PI3 kinase p110 subunit alpha
    • PI3 kinase regulatory subunit alpha
    • PI3 kinase subunit alpha
    • PI3 kinase subunit p85 alpha
    • PI3K
    • PI3K alpha
    • PI3K regulatory subunit alpha
    • PIK3CA
    • PIK3R1
    • PtdIns 3 kinase p110
    • PtdIns 3 kinase regulatory subunit alpha
    • PtdIns 3 kinase regulatory subunit p85 alpha
    • PtdIns 3 kinase subunit alpha
    • PtdIns 3 kinase subunit p110 alpha
    see all
  • 関連性Phosphoinositide 3-kinases (PI3Ks) phosphorylate phosphatidylinositols (PIs) at their 3´ OH position generating lipid second messengers and thereby regulate numerous biological processes including cell growth, differentiation, survival, proliferation, migration and metabolism. On the basis of structural similarities and substrate specificity, the PI3K family can be subdivided into three classes termed I, II, and III. All human class I members are heterodimers consisting of a catalytic subunit (MW approx. 110 kDa) and a non-catalytic subunit (MW 50, 55, 85, or 101 kDa) and are known to phosphorylate phosphatidylinositol (PI), phosphatidylinositol-4-mono-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2) in vitro. The class I members can be further subdivided into class IA and IB PI3Ks. Class IA exists in three isoforms (p110alpha, p110beta and p110delta whereas the only class IB member is termed p110gamma. Class IA PI3Ks are activated by adaptor proteins such as Ras or BCAP, or tyrosine-kinase-associated receptors including antigen, co-stimulatory and cytokine receptors (e.g. CD19, CD28, Insulin receptor, EGFR, and PDGFR). p110gamma is activated by G-protein-coupled receptors (GPCRs). Effectors of class I PI3Ks are pleckstrin homology domain proteins such as Akt/PKB, BTK, TEC, ITK, BAM32, and small GTPases (e.g. Cdc42, Rac, or Ras). The action of PI3Ks is regulated by the phosphatidylinositol- 3,4,5-trisphosphate phosphatases SHIP and PTEN.

Recombinant human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein 画像

  • Sample kinase activity plot for ab91093.
  • SDS-PAGE showing ab91093.

Recombinant human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein (ab91093) 使用論文

ab91093 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"