製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 配列
      MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK
    • 分子量
      31 kDa including tags
    • 領域
      1 to 254
    • タグ
      His tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab100885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Mass Spectrometry

    SDS-PAGE

    Western blot

  • 質量分析
    MALDI-TOF
  • 精製度
    > 90 % SDS-PAGE.
    ab100885 is purified using conventional chromatography techniques.
  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: 0% None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

関連情報

  • 別名
    • BPG dependent PGAM 1
    • BPG dependent PGAM1
    • BPG-dependent PGAM 1
    • OTTHUMP00000059414
    • PGAM 1
    • PGAM A
    • PGAM B
    • PGAM-B
    • PGAM1
    • PGAM1_HUMAN
    • PGAMA
    • PGAMB
    • Phosphoglycerate mutase 1
    • Phosphoglycerate mutase 1 brain
    • Phosphoglycerate mutase A
    • Phosphoglycerate mutase A nonmuscle form
    • Phosphoglycerate mutase isozyme B
    see all
  • 機能
    Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.
  • 組織特異性
    In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues.
  • 配列類似性
    Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
  • Information by UniProt

画像

  • ab100885 (3 µg) on an SDS-PAGE gel (15%).
  • All lanes : Anti-PGAM1 antibody (ab96622) at 1/1000 dilution

    Lane 1 : Recombinant Human PGAM1 protein (ab100885) at 0.1 µg
    Lane 2 : Recombinant Human PGAM1 protein (ab100885) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

参考文献

ab100885 has not yet been referenced specifically in any publications.

レビューと Q&A

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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