Recombinant human Peroxiredoxin 1/PAG protein (ab79945)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
-
製品名
Recombinant human Peroxiredoxin 1/PAG protein
Peroxiredoxin 1/PAG タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity between 800 and 1000 pmol/min/µg. Please enquire as to activity of specific lots. 1.88 µg of Peroxiredoxin 1 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.
-
精製度
> 90 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected insect cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab79945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Western blot
Functional Studies
SDS-PAGE
-
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- Heme binding 23 kDa protein
- MSP23
- Natural killer cell-enhancing factor A
see all -
機能
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. -
配列類似性
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
翻訳後修飾
Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. -
細胞内局在
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab79945 は 1 報の論文で使用されています。
- Booty LM et al. Selective Disruption of Mitochondrial Thiol Redox State in Cells and In Vivo. Cell Chem Biol 26:449-461.e8 (2019). PubMed: 30713096