Recombinant human PERK protein (ab101115)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human PERK protein -
生理活性
The specific activity of ab101115 was determined to be 18 nmol/min/mg by activity assay.
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精製度
> 90 % SDS-PAGE.
The purity was determined to be >90% by densitometry -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
NKYDSVSGEANDSSWNDIKNSGYISRYLTDFEPIQCLGRGGFGVVFEAKN KVDDCNYAIKRIRLPNRELAREKVMREVKALAKLEHPGIVRYFNAWLEAP PEKWQEKMDEIWLKDESTDWPLSSPSPMDAPSVKIRRMDPFATKEHIEII APSPQRSRSFSVGISCDQTSSSESQFSPLEFSGMDHEDISESVDAAYNLQ DSCLTDCDVEDGTMDGNDEGHSFELCPSEASPYVRSRERTSSSIVFEDSG CDNASSKEEPKTNRLHIGNHCANKLTAFKPTSSKSSSEATLSISPPRPTT LSLDLTKNTTEKLQPSSPKVYLYIQMQLCRKENLKDWMNGRCTIEERERS VCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAM DQDEEEQTVLTPMPAYARHTGQVGTKLYMSPEQIHGNSYSHKVDIFSLGL ILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCEYVMVQDMLSPSP MERPEAINIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKHSRQSNNSHSP LPSN -
予測される分子量
115 kDa including tags -
領域
563 to 1115
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特性
Our Abpromise guarantee covers the use of ab101115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
ab61428 (Human Smad3 full length protein) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- DKFZp781H1925
- E2AK3_HUMAN
- EC 2.7.11.1
see all -
機能
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1. -
組織特異性
Ubiquitous. A high level expression is seen in secretory tissues. -
関連疾患
Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 1 protein kinase domain. -
ドメイン
The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP. -
翻訳後修飾
Autophosphorylated.
N-glycosylated. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
画像
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The specific activity of PERK (ab101115) was determined to be 16 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab101115
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SDS PAGE analysis of ab101115
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The putity of ab101115 was determined to be 90% by densitometry.
Approximate MWt: 115kDa -
The specific activity of ab101115 was determined to be 18 nmol/min/mg by activity assay.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab101115 は論文での使用が確認できていません。