Recombinant Human Perforin protein (ab112345)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Wheat germ
  • アミノ酸配列
    • アクセッション番号P14222
    • 生物種Human
    • 配列WSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRC NLNHGHLKFRYHARCLPHLGGGTCLDYVPQMLLGEPPGNRSGAVW
    • 分子量36 kDa including tags
    • 領域461 to 555

特性

Our Abpromise guarantee covers the use of ab112345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性useful for Antibody Production and Protein Array
  • アプリケーション

    SDS-PAGE

    ELISA

    Western blot

  • 製品の状態Liquid
  • 備考useful for Antibody Production and Protein Array
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl
    Note: Glutathione is reduced

関連情報

  • 別名
    • Cytolysin
    • FLH2
    • HPLH2
    • Lymphocyte pore-forming protein
    • P1
    • PERF_HUMAN
    • Perforin 1
    • perforin 1 (pore forming protein)
    • Perforin-1
    • PFP
    • PGFL
    • PIGF
    • PIGF-2
    • PLGF
    • Pore forming protein
    • prf1
    • SHGC-10760
    see all
  • 機能Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
  • 関連疾患Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
  • 配列類似性Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 C2 domain.
    Contains 1 EGF-like domain.
    Contains 1 MACPF domain.
  • ドメインThe C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
  • Information by UniProt

Recombinant Human Perforin protein 画像

  • 12.5% SDS-PAGE showing ab112345 at approximately 36.08kDa stained with Coomassie Blue.

Recombinant Human Perforin protein (ab112345) 使用論文

ab112345 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab112345.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"