製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human

特性

Our Abpromise guarantee covers the use of ab60834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific Activity: 123 nmol/min/mg.
  • アプリケーション

    Functional Studies

    SDS-PAGE

    Western blot

  • 製品の状態Liquid
  • 備考

    ab204873 (AKT1 + PKN2 peptide substrate) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, 0.1mM PMSF, pH 7.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • 3 phosphoinositide dependent protein kinase 1
    • 3-phosphoinositide-dependent protein kinase 1
    • hPDK 1
    • hPDK1
    • MGC20087
    • MGC35290
    • OTTHUMP00000159109
    • OTTHUMP00000159110
    • OTTHUMP00000174525
    • PDK1
    • Pdpk1
    • PDPK1_HUMAN
    • PDPK2
    • PkB kinase
    • PkB kinase like gene 1
    • PkB like 1
    • PRO0461
    • Protein kinase
    see all
  • 機能Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • 組織特異性Appears to be expressed ubiquitously.
  • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • 細胞内局在Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Information by UniProt

Recombinant human PDPK1 protein 画像

  • Sample Kinase Activity Plot.
  • ab60834 on SDS-PAGE, MW ~67kDa.
  • Anti-PDPK1 antibody (ab31406) at 1/1000 dilution + Recombinant human PDPK1 protein (ab60834) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Recombinant human PDPK1 protein (ab60834) 使用論文

ab60834 has not yet been referenced specifically in any publications.

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