製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 領域
      11 to 411
    • タグ
      His tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab110356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    PDK4 activity was measured in a serial dilution of the recombinant protein and the minimum amount of the protein required to phosphorylate and fully inhibit PDH immunocaptured from given amount of human, bovine, rat or mouse samples was determined.
  • アプリケーション

    Functional Studies

    Sandwich ELISA

  • 精製度
    > 90 % Purified via His tag.
    PDK4 was purified by metal chelation column chromatography (Ni-IDA) using His Binding Columns.
  • 製品の状態
    Liquid
  • 備考

    PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.

    Product was previously marketed under the MitoSciences sub-brand.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.14% Sodium phosphate, 1.46% Sodium chloride, 30% Glycerol, 0.08% DTT, 0.02% EDTA, 0.05% Triton-X-100

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4
    • FLJ40832
    • mitochondrial
    • Pdk4
    • PDK4_HUMAN
    • Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial
    • Pyruvate dehydrogenase kinase 4
    • Pyruvate dehydrogenase kinase isoenzyme 4
    • Pyruvate dehydrogenase kinase isoform 4
    • Pyruvate dehydrogenase kinase isozyme 4
    • Pyruvate dehydrogenase kinase isozyme 4 mitochondrial
    see all
  • 機能
    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • 組織特異性
    Ubiquitous; highest levels of expression in heart and skeletal muscle.
  • 配列類似性
    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • 細胞内局在
    Mitochondrion matrix.
  • Information by UniProt

参考文献

ab110356 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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