Recombinant human PDGF CC protein (ab84066)

製品の概要

  • 製品名Recombinant human PDGF CC protein
  • タンパク質長Protein fragment

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列MVVDLNLLTE EVRLYSCTPR NFSVSIREEL KRTDTIFWPG CLLVKRCGGN CACCLHNCNE CQCVPSKVTK KYHEVLQLRP KTGVRGLHKS LTDVALEHHE ECDCVCRGST GG

特性

Our Abpromise guarantee covers the use of ab84066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Biological Activity : Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 15-20 ng/ml.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • エンドトキシン・レベル< 0.100 Eu/µg
  • 精製度> 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE gel and HPLC analyses.
  • 製品の状態Lyophilised
  • 備考Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Reconstituted PDGF CC is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.

関連情報

  • 別名
    • FALLOTEIN
    • PDGF C
    • PDGFC
    • Platelet derived growth factor
    • platelet-derived growth factor C
    • SCDGF
    • secretory growth factor-like protein
    • Spinal cord-derived growth factor
    • VEGF E
    see all
  • 関連性The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14 kDa polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-alpha and PDGFR-beta, and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in platelet alpha-granules and are released upon platelet activation. PDGF-AA, -AB, -BB and –CC signal primarily through the PDGF R alpha receptor, whereas PDGF-DD interacts almost exclusively with the PDGF R beta receptor.
  • 細胞内局在Cytoplasmic, Nuclear, Secreted and Cytoplasmic granule

Recombinant human PDGF CC protein (ab84066) 使用論文

ab84066 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam regarding ab84066. I have confirmed the following reconstitution instructions with the lab: - Centrifuge the vial prior to opening. - Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. - T...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"