製品の概要

法規制情報

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種
      Human
    • タグ
      GST tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab54113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    Specific Activity: 97 U/ug. 1 unit will release 1 pmole phosphate per minute from 400 uM 5’-GMP at 30 °C in a reaction buffer of 10 mM Tris-HCl, pH 7.4, 1 mM MgCl2. Assay buffer: 10mM Tris-HCl, pH 7.4, 1 mM Magnesium chloride ; 3’, 5’-cGMP substrate: 0.4 mM; PDE9A: 5 ng/ul.
  • アプリケーション

    SDS-PAGE

    Phosphatase Activity

    Inhibition Assay

  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 5mM Glutathione, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • 5''-cyclic phosphodiesterase 9A
    • cGMP specific 3' 5' cyclic phosphodiesterase type 9
    • FLJ90181
    • High affinity cGMP-specific 3''
    • High-affinity cGMP-specific 3'5'-cyclic phosphodiesterase 9A
    • HSPDE9A2
    • OTTHUMP00000109399
    • PDE 9A
    • Pde9a
    • PDE9A_HUMAN
    • Phosphodiesterase 9A
    • phosphodiesterase PDE9A21
    see all
  • 機能
    Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.
  • 組織特異性
    Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate.
  • 配列類似性
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily.
  • ドメイン
    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
  • 細胞内局在
    Cytoplasm. Endoplasmic reticulum; Cell projection > ruffle membrane. Cytoplasm > perinuclear region and Cell projection > ruffle membrane. Cytoplasm > perinuclear region. Golgi apparatus. Endoplasmic reticulum.
  • Information by UniProt

参考文献

ab54113 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab54113.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録