Recombinant human PDE5A/PDE5 protein (ab125581)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
-
製品名
Recombinant human PDE5A/PDE5 protein
PDE5A/PDE5 タンパク質・ペプチド 製品一覧 -
生理活性
The specific activity of ab125581 was determined to be 2275 nmol/min/mg.
-
精製度
> 90 % Densitometry.
The purity was determined to be 95% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
予測される分子量
63 kDa including tags -
領域
537 to 875 -
タグ
GST tag N-Terminus
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab125581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
SDS-PAGE
-
製品の状態
Liquid -
備考
This product was previously labelled as PDE5A
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.002% PMSF, 0.5% Magnesium chloride, 0.003% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- 5''-cyclic phosphodiesterase
- CGB PDE
- CGB-PDE
see all -
機能
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. -
組織特異性
Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung. -
パスウェイ
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. -
配列類似性
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
ドメイン
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B. -
翻訳後修飾
Phosphorylation is regulated by binding of cGMP to the two allosteric sites. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab125581 は 1 報の論文で使用されています。
- Chau Y et al. Exploration of icariin analog structure space reveals key features driving potent inhibition of human phosphodiesterase-5. PLoS One 14:e0222803 (2019). PubMed: 31539416