製品の概要

法規制情報

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 分子量
      110 kDa including tags
    • 領域
      332 to 886
    • タグ
      GST tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab125603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    The specific activity was determined to be 1082 nmol/min/mg
  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 精製度
    > 95 % Densitometry.

  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • 5''-cyclic phosphodiesterase 4A
    • cAMP specific phosphodiesterase
    • cAMP-specific 3''
    • cAMP-specific 3',5'-cyclic phosphodiesterase 4A
    • DPDE2
    • dunce like phosphodiesterase E2
    • PDE 4A
    • PDE4
    • PDE46
    • PDE4A
    • PDE4A_HUMAN
    • PDE4A11
    • Phosphodiesterase 4A
    • phosphodiesterase 4A cAMP specific
    • Phosphodiesterase 4A, cAMP-specific (dunce
    • phosphodiesterase E2 dunce homolog
    • Phosphodiesterase E2 dunce homolog, Drosophila
    • phosphodiesterase isozyme 4
    • phosphodiesterase type 4A11
    see all
  • 機能
    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • 組織特異性
    Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
  • パスウェイ
    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • 配列類似性
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • 翻訳後修飾
    Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
    Proteolytically cleaved by caspase-3.
  • 細胞内局在
    Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
  • Information by UniProt

Recombinant human PDE4A protein 画像

Recombinant human PDE4A protein (ab125603) 使用論文

ab125603 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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