Recombinant Human PCM1 protein (ab114699)
Key features and details
- Expression system: Wheat germ
- Suitable for: ELISA, SDS-PAGE, WB
製品の詳細
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製品名
Recombinant Human PCM1 protein -
発現系
Wheat germ -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKK KFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAEL EKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINT NKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAM ESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQE KSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTT ARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAE QAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAV PDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMD KLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSL TSSVPYPTASLVSQNESENEGHLNPSEKLQ -
予測される分子量
84 kDa including tags -
領域
1 to 530
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特性
Our Abpromise guarantee covers the use of ab114699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
ELISA
SDS-PAGE
Western blot
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製品の状態
Liquid -
備考
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.3% Glutathione, 0.79% Tris HCl
関連情報
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別名
- hPCM-1
- PCM-1
- pcm1
see all -
機能
Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia. -
組織特異性
Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid. -
関連疾患
Defects in PCM1 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving PCM1 is found in thyroid papillary carcinomas. Translocation t(8;10)(p21.3;q11.2) with RET links the protein kinase domain of RET to the major portion of PCM1.
Note=A chromosomal aberration involving PCM1 is found in a variety of hematological malignancies including atypical chronic myeloid leukemia (atypical CML) and T-cell lymphoma. Translocation t(8;9)(p22;p24) with JAK2 links the protein kinase domain of JAK2 to the major portion of PCM1. -
配列類似性
Belongs to the PCM1 family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic granule. Localizes to cytoplasmic granules which are enriched around the centrosome. This centrosomal enrichment requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab114699 は論文での使用が確認できていません。