Recombinant Human PC4 (mutated F77P) protein (ab82047)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: Functional Studies, SDS-PAGE, GSA
製品の詳細
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製品名
Recombinant Human PC4 (mutated F77P) protein
PC4 タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
ab82047 is purified by conventional chromatography. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
修飾
mutated F77P
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab82047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
Gel Supershift Assays
EMSA
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製品の状態
Liquid -
備考
A single amino acid change at position 77 (F to P) abolishes both ds- and ss-DNA binding activity.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.9
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)
関連情報
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別名
- Activated RNA polymerase II transcriptional coactivator p15
- Interferon related developmental regulator 1
- MGC102747
see all -
機能
General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA). -
配列類似性
Belongs to the transcriptional coactivator PC4 family. -
翻訳後修飾
Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region. -
細胞内局在
Nucleus. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab82047 は論文での使用が確認できていません。