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Our Abpromise guarantee covers the use of ab75605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Reconstituted PARP1 will retain residual polyADP-ribosylation enzymatic activity.
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents (upon reconstitution in 100ul distilled water): 50mM Tris, 100mM Sodium chloride, 10mM Magnesium chloride, 1mM DTT, 1mM NAD , 10 µg/mL activated DNA, plus lyophilization stabilizers. pH 8.
Lane A: unmodified PARP1 LANE B: automodified PARP1 Lane B shows a smear of staining above MW 113kDa indicating polyADP-ribosylated PARP1 modified to various extents. A significant amount of automodified PARP1 did not enter the gel (top arrow). This image shows that most of the PARP1 will be at the top of the gel with a smear running up from 113kDa indicating PARP1 is automodified to various extents. There is little or no native PARP1 at 113kDa.
No staining is visible in Lane 1 (unmodified PARP1). Lane 2 shows a smear above M.Wt 113 kda indication poly ribosylated PARP1 modified to various extents.
ab75605 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"