The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity: 2.1 nmoles/min/µg. Analysis of enzymatic activity: 1. Different concentration of PAK6 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 20) at room temperature for 1 hour. 2. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT. 3. Fluorescence was detected using ?exc=460±40 nm and ?em=528±20 nm filters.
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Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.