製品の概要

法規制情報

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 分子量
      95 kDa including tags
    • 領域
      1 to 545

特性

Our Abpromise guarantee covers the use of ab125805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    The specific activity of ab125805 was determined to be 68 - 74 nmol/min/mg.
  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 精製度
    > 95 % Densitometry.
    Purity was determined to be >95% by densitometry.
  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • ADRB2
    • Alpha PAK
    • Alpha-PAK
    • MGC130000
    • MGC130001
    • p21 activated kinase 1
    • p21 protein (Cdc42/Rac) activated kinase 1
    • p21-activated kinase 1
    • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related)
    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
    • p65 PAK
    • p65-PAK
    • P68-PAK
    • PAK alpha
    • PAK-1
    • Pak1
    • PAK1_HUMAN
    • Paka
    • PAKalpha
    • Protein kinase MUK2
    • Rac/p21-activated kinase
    • Serine/threonine-protein kinase PAK 1
    • STE20 homolog yeast
    see all
  • 機能
    The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • 配列類似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Autophosphorylated when activated by CDC42/p21 and RAC1.
  • 細胞内局在
    Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
  • Information by UniProt

画像

  • SDS-PAGE analysis of ab125805.
  • Sample Kinase Assay showing the specific activity of ab125805 to be 68 nmol/min/mg.

参考文献

ab125805 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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