Recombinant human PAI1 (mutated S338) protein (ab92773)

製品の概要

• 製品名Recombinant human PAI1 (mutated S338) protein
  PAI1 タンパク質・ペプチド 製品一覧
• タンパク質長Full length protein

製品の詳細

• 由来Recombinant
• 由来Escherichia coli
• アミノ酸配列
  • 生物種Human
  • 修飾mutated S338

特性

• 生理活性Specific activity: > 95 percent active by uPA titration; 1 mole NBD / mole PAI1. Excitation: 490 nm; Emission profile: 520 nm – 600 nm. Binding of uPA or tPA results in increase of peak at 540 nm.
• アプリケーション

  Functional Studies

  SDS-PAGE

• 精製度> 95 % SDS-PAGE.
  ab92773 is > 98% pure by SDS-PAGE.
  
• 製品の状態Liquid
• 備考Protect from light.
  Solubility: > 2 mg/mL and < 5 mg/mL

  
  

  ab92773 was created by mutagenesis of the P9 serine residue (Ser338) on the reactive center loop to cysteine. This then provided a free thiol group for incorporation of N,N'-dimethyl-N-(acetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) (NBD), a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P9-NBD PAI1 is enhanced 6-7 fold upon insertion of the reactive center loop into beta-sheet A following complex formation with proteinases, formation of the latent species, or cleavage by elastase. The incorporated probe is excited at 480 nm and displays a broad emission spectrum with a peak centered 542 nm with a resultant blue-shift to 520 nm following reactive center loop insertion. The modified PAI1 is nearly as active as wt PAI-1 and is more resistant to the spontaneous latency reaction making this an excellent tool for monitoring reaction rates of PAI1. ab92773 has been utilized in a number of studies to determine the rates of loop insertion and SERPIN reaction mechanisms when reacted with various proteinases, inactivating antibodies and conformational changes imposed by the binding of vitronectin.

• Concentration information loading...

前処理および保存

• 保存方法および安定性

  Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

  Preservative: None
  Constituents: 0.1M Sodium chloride, 0.05M Sodium phosphate, 1mM EDTA, pH 6.6

  This product is an active protein and may elicit a biological response in vivo, handle with caution.

Recombinant human PAI1 (mutated S338) protein 画像

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  Functional Studies - PAI1 protein (Mutated S338) (Active) (ab92773)
  Binding of uPA or tPA results in increase of peak at 540 nm.