Recombinant Human PAI1 (mutated ) protein (ab93232)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: Inhibition Assay, SDS-PAGE
製品の詳細
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製品名
Recombinant Human PAI1 (mutated ) protein
PAI1 タンパク質・ペプチド 製品一覧 -
生理活性
A triple mutation on the wild-type background makes ab93232 selectively inhibit tPA. Urokinase inhibition is limited to 1/25,000 of tPA inhibition by the P3Tyr-P2Ser-P1Tyr mutation. -
精製度
> 95 % SDS-PAGE. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
修飾
mutated P1Y + P2S + P3Y
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特性
Our Abpromise guarantee covers the use of ab93232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Inhibition Assay
SDS-PAGE
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製品の状態
Liquid -
備考
A triple mutation on the wild-type background makes ab93232 selectively inhibit tPA. Urokinase inhibition is limited to 1/25,000 of tPA inhibition by the P3Tyr-P2Ser-P1Tyr mutation. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 6.60
Constituents: 0.82% Sodium phosphate, 0.0292% EDTA, 0.58% Sodium chloride
関連情報
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別名
- Clade E
- Endothelial plasminogen activator inhibitor
- Nexin
see all -
機能
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. -
組織特異性
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
関連疾患
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
配列類似性
Belongs to the serpin family. -
翻訳後修飾
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab93232 は論文での使用が確認できていません。