Recombinant human PAI1 (mutated N150 H + K154 T + Q319 L + M354 I) protein (ab92969)



  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human


Our Abpromise guarantee covers the use of ab92969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性>95% active by uPA titration. Kinetic Data: Second order rate constants for inhibition of: uPA = 5.1 X 106 M-1s-1 tPA = 7.9 X 105 M-1s-1
  • アプリケーション


    Functional Studies

  • 精製度> 95 % SDS-PAGE.
    > 99% pure by SDS PAGE
  • 製品の状態Liquid
  • 備考Solubility: > 2 mg/mL and < 5 mg/mL Ultraviolet: Absorbance (280nm) = 2.5 epsilon 0.1% = 1.0

    ab92969 contains the following four mutations: K154T, Q319L, M354I and N150H. These mutations combine to confer great stability to the otherwise labile molecule essentially locking in into the active conformation. It is an ideal choice for in vivo studies.

  • Concentration information loading...


  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Sodium phosphate, 100mM Sodium chloride, 1mM EDTA, pH 6.6

    This product is an active protein and may elicit a biological response in vivo, handle with caution.


  • 別名
    • Clade E
    • Endothelial plasminogen activator inhibitor
    • Nexin
    • PAI
    • PAI 1
    • PAI-1
    • PAI1_HUMAN
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Plasminogen activator inhibitor type 1
    • Serine (or cysteine) proteinase inhibitor
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpin E1
    • Serpin peptidase inhibitor clade E
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpine 1
    • SERPINE1
    see all
  • 機能This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • 組織特異性Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 関連疾患Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 配列類似性Belongs to the serpin family.
  • 翻訳後修飾Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 細胞内局在Secreted.
  • Information by UniProt

Recombinant human PAI1 (mutated N150 H + K154 T + Q319 L + M354 I) protein 画像

  • 10% SDS Page Gelcode Blue Stain
    1. 3ug ab92969 reduced
    2. 3ug ab92969 + 12ug Urokinase reduced
    3. prestained standard

Recombinant human PAI1 (mutated N150 H + K154 T + Q319 L + M354 I) protein (ab92969) 使用論文

ab92969 has not yet been referenced specifically in any publications.

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