製品の概要

  • 製品名Recombinant Human PAG protein
  • タンパク質長Protein fragment

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 領域41 to 432

特性

Our Abpromise guarantee covers the use of ab56521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Western blot

  • 精製度> 95 % SDS-PAGE.
    Purified by Immobilized Metal Affinity Chromatography used for purification of polyhistidine-tagged recombinant proteins. > 95% (by SDS-PAGE)
  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 100mM Sodium chloride, 10mM Sodium phosphate, pH 8

  • 再構成Reconstitute with 100 ul sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.

関連情報

  • 別名
    • CBP
    • Csk binding protein
    • Csk-binding protein
    • FLJ37858
    • MGC138364
    • PAG
    • PAG 1
    • PAG1
    • PAG1_HUMAN
    • Phosphoprotein associated with glycosphingolipid enriched microdomains
    • Phosphoprotein associated with glycosphingolipid enriched microdomains 1
    • Phosphoprotein associated with glycosphingolipid microdomains 1
    • Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
    • Protein Associated with Glycosphingolipid Enriched Microdomains
    • Transmembrane adapter protein PAG
    • Transmembrane phosphoprotein Cbp
    see all
  • 機能Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
  • 組織特異性Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
  • 翻訳後修飾Palmitoylated.
    Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
  • 細胞内局在Cell membrane. Present in lipid rafts.
  • Information by UniProt

Recombinant Human PAG protein 画像

  • SDS PAGE analysis of ab56521: 12% separating gel, 6% stacking gel; reducing conditions. Staining with Coomasie Blue. Lane 1-5: PAG recombinant protein (2 µg, 1 µg, 0.5 µg, 0.2 µg, 0.1 µg per lane).
  • Western Blot analysis of ab56521. Reducing conditions. Lane 1-4: immunostaining with specific anti PAG antibody. Detection with Goat anti-rabbit / HRP secondary antibody. Amount of PAG recombinant protein loaded on each lane: (1) 0.1 µg, (2) 0.050 µg, (3) 0.020 µg, (4) 0.010 µg.

Recombinant Human PAG protein (ab56521) 使用論文

This product has been referenced in:
  • Le Page A  et al. Downregulation of inhibitory SRC homology 2 domain-containing phosphatase-1 (SHP-1) leads to recovery of T cell responses in elderly. Cell Commun Signal 12:2 (2014). WB ; Human . Read more (PubMed: 24405902) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"